Distribution of cytolytic activity in cultures of Gomphosphaeria aponina¹

Abstracted in part from a dissertation submitted by L. F. McCoy, Jr. in partial fulfillment of the requirements for the degree of doctor of philosophy, University of South Florida, December 1977.

Abstract

Cultures of the blue‐green alga, Gomphosphaeria aponina, have been subjected to continuous cell centrifugation, and the distribution of cytolytic activity has been evaluated. Extracts of the cultures are cytolytic toward a red tide organism, Gymnodinium breve. Improved cytolytic assay procedures are described. Most of the cytolytic activity (60%) is associated with the chloroform extracts of the cell‐free medium, and the agreement successive experiments is good (e.g., 64 vs. 59–61% of total activity in the cell‐free extracts).

Key words:

• aponin
• cytolytic activity
• distribution
• Gymnodinium breve
• Gomphosphaeria aponina
• red tide.

Notes

Abstracted in part from a dissertation submitted by L. F. McCoy, Jr. in partial fulfillment of the requirements for the degree of doctor of philosophy, University of South Florida, December 1977.

Present address: Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas 66103.