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Abstract
The microbially mediated disappearance of 1,3‐dinitrobenzene was complete in Tennessee River water samples taken downstream from a munitions production facility but not in local (Frederick, MD) water samples. Microorganisms from the Tennessee River could be grown on 1,3‐dinitrobenzene as a sole carbon source and could mineralize the compound. At 106 total microorganisms per mL and 25°C, the half‐life of 1,3‐dinitrobenzene in Tennessee River samples and enrichment cultures was estimated to be 1 and 9.7 days, respectively. Growth of Tennessee River microorganisms on 1,3‐dinitrobenzene did not result in their metabolism of several other nitroaromatic compounds found in munitions discharges.