Interactions among mononitrophenol isomers during biodegradation of their mixtures

Abstract

Continuous aerobic biodegradation of 4-NP, 3-NP and 2-NP mixture was monitored in a packed bed reactor in simulated wastewater with a mixed microbial culture immobilized on expanded slate. Substrate loading was varied by increasing the concentration of one isomer while keeping the other two at constant levels, all at a constant residence time of 60 min. At large concentrations, all of the individual NP isomers suppressed the degradation rates of the other isomers at steady state; however, the observed patterns and threshold concentrations were different for all three substrates. As a result, conditions were determined for stable and efficient removal of NP mixtures. Changes of the biofilm composition during a long-term operation were identified.

Keywords:

• Biofilm composition
• nitrophenol mixtures
• packed bed reactor
• wastewater treatment

Funding

The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic, Project KONTAKT II - LH13073.