In vivo effects of aflatoxin B1 and benzo[a]pyrene on the heart muscle of chicken embryos

Abstract

The purpose of the present study was to evaluate the effects of aflatoxin B1 (AFB1) and benzo[a]pyrene (BaP) on the heart muscle of chicken embryos of the broiler line Ross 308. The benzo[a]pyrene in the organic oil solution was injected in ovo on the 6th day of the incubation in doses of: 0.1, 0.5, and 1 mg/kg weight of eggs; the aflatoxin B1 in the organic oil solution was injected in ovo on the 6th day of the incubation into the yolk in doses of 80, 120 and 240 ng/kg weight of eggs. Multiple biochemical and hepatic parameters have been observed, including sodium, potassium, chloride, cholesterol, uric acid, total proteins, aminotransferase aspartate, and aminotransferase alanine. A low dose of AFB1 and BaP administered in ovo during early embryonic development had a significant impact on chicken embryonic development, as demonstrated by alterations in biochemical, mineral, and hepatic parameters.

Keywords:

• Aflatoxin B1
• Benzo[a]pyrene
• Heart
• Embryonated eggs
• Ross 308

Disclosure statement

No potential conflict of interest was reported by the authors.

Data availability statement

1. The datasets generated during and/or analyzed during the current study are available in the Mendeley repository, https://data.mendeley.com//datasets/tx3tw29p62/1. .

2. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

3. All data generated or analyzed during this study are included in this published article (and its supplementary information files).

Additional information

Funding

This work was financially supported by projects APVV-15-0543, APVV-16-0289, APVV-20-0218, VEGA 1/0038/19, VEGA 1/0539/18, and VEGA 1/0083/21.