Extracellular microbial lipids from pseudomonas S_p LS₁ as bioemulsifiers

Abstract

Microbes have been isolated from contaminated soil and wastewater by enrichment culture experiments for bioemulsifier production and cell free broth was tested for bioemulsifier by measuring surface tension, emulsifying activity. One of the strains, Pseudomonas s_p LS₁, was studied in detail for the production of bioemulsifier from different carbon sources for optimization of substrate concentration, its application and characterization. Pseudomonas s_p LS₁ is capable of reducing surface tension of glucose containing broth from 55 to 30 mN/m. The bioemulsifier production was growth associated. Preliminary structure identification with specific thin layer chromatography agent (CH₃CI₃ : MeOH : H₂o :j 69 : 24 : 2) extracts showed, one spot having rhamnolipid structure with Rf value of 0.54. The rhamnolipid production by Pseudomonas Sp LS₁ may have potential for facilitating the bioremediation of contaminated sites with water insoluble aromatics.

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