In vivo fluorimetric assay of DNA unwinding as evidence of the damage induced by folpet in rat liver DNA

Abstract

Folpet, metalaxyl, and procymidone were tested as pure active ingredients for induction of single‐strand breaks in liver DNA after intraperitoneal (i.p.) injection into rats. Folpet was found to be capable of inducing DNA damage because it significantly increased the DNA unwinding rate (measured by fluorimetric assay following administration of dosages which were unable to induce an initial degree of centrilobular liver degeneration). On the other hand, metalaxyl and procymidone were determined not to damage rat liver DNA.