Abstract
Experimental investigations were carried out to determine the methodology and optimum conditions to extract insulin hormone from the pancreas glands collected from the slaughterhouse wastes existing in the United Arab Emirates.
The pancreas glands of sheep, cattle and camels were separately trimmed from fatty and non pancreatic tissues, frozen, rotoground, macerated and finally homogenized. The homogenized pancreas glands were extracted at a temperature of 20 ± 1°C using a mechanical extraction operation for an extraction time of 2 hours, by the extracting solvent which is 80% by weight ethyl alcohol acidified by phosphoric acid in a ratio of 30 grams/kg. to bring the pH value of the solution to 3.
The maximum yield of the extracted insulin hormone was obtained when the solvent to feed weight ratio is more than 350:100.
In the case of the homogenized pancreas glands of sheep, cattle and camels the yield of the extracted insulin hormone reached to 0.783, 0.816 and 0.922 grams of insulin hormone per 100 grams of the homogenized glands respectively.
The proposed scheme of the industrial production of insulin hormone from pancreas glands available in the slaughterhouse wastes existing in the UAE is discussed in detail.
Notes
All correspondence should be addressed to Dr. Gamil Radwan, P.O. Box 1915, Al‐Ain City, United Arab Emirates