The effects of some pesticides on human and bovine erythrocyte carbonic anhydrase enzyme activities in vitro

Abstract

The influence of Penncozeb [Mangan‐zinc ethylenediamine‐bisdithicarbamate], Dithane, [Manganese etylenebisdithiocarbamate], Cupravit [Cuper oksichloride], Bayleton [1‐(4‐ chlorophenoxy)‐3,3‐dimethyl‐1‐(1H‐1.2.4‐Thiazol‐l‐yl)‐2.2‐butanon], Baythroid [Cyano‐(4 fluoro‐3‐phenoxyphenyl)‐methyl‐3‐(2.2‐dichloroethyl ‐2. 2 metyl ‐ cyclo‐propancarboxylate], Mavrik [‐cyano‐3‐phenoxybenzyl N‐(2‐chloro—trifluoro ‐ p ‐tolyl], [alstar [2‐methyl biphenyl‐3‐ylmethyl(z)‐(IRS,3RS)‐3‐(2‐chloro‐3.3.3 ‐triflouroprop ‐1‐enyl)=Dimethylcyclo propenecarboxyate], Endosulfan [6.7.8.9.10.10‐hekzachloro‐1.5.5a.6.9.9a ‐ hekzahydro‐6. 9‐methano‐2.3.4 ‐ benzole dioxi atehiepin 3‐oxide] has been investigated on human erythrocyt carbonic anhydrase isozymes (HCA‐I, HCA‐II) and bovine erythrocyte carbonic anhydrase (BCA) in vitro. Among the chemicals; Baythroid, Talstar, Mavrik were determined to have inhibition effect, on bovine CA and human CA isoenzymes. Dithane had effect of inhibition only on BCA. The I₅₀ values of chemicals caused inhibition were determined by means of activity percentage [I] diagrams. The values were 2.77×10^‐3 M. 3.8×10^‐3 M, 1.56×10^‐2 M, 2.36×10^‐2 M for BCA, respectively. The values of same chemicals except dithane were 1.67×10^‐3 ?, 4.64×10^‐3 M, 3.9×10^‐3M for HCA‐I, and 3.16×10^‐2M, 2.81×10^‐3 M, 1.04×l0^‐2 M for HCA‐II, respectively. On the other hand, Changes occurred in stock concentration of chemicals showed activator effect were calculated. According to the results reached, 10^‐2 M cupravit, 4.17×10^‐2 M endosulfan and 10^‐2 M bayleton in stock concentration activated on both BCA and HCA‐II at different levels. The values activation increases of these chemicals on BCA were 29.90, 46.40, 43.70 %, and 67.11, 57.23, 31.88 % for HCA‐II, respectively. Cupravit and bayleton which activated to BCA and HCA‐II were ineffective on HCA‐I. Values of endosulfan was determined to be 114.40 %. Penncozeb was recorded ineffective for all isozymes.

Key words:

• Purification
• Carbonic anhydrase
• Pesticides