The inhibition effects of some pesticides on the in vitro activity of five serum enzymes

Abstract

The effect of some pesticides on alkaline phosphatase (ALK‐P) ( EC. 3.1.3.1 ) , Amylase ( E.C. 3.2.1.1 ) creatine kinase ( CK ) ( E.C. 2.7.3.2 ), δ‐glutamyltransferase ( GGT‐P ) ( E.C. 2.3.2.1 ) and lactate dehydrogenase ( LDH ) (E.C. 1.1.1.27) were investigated. These pesticides were baytroid [Cyano ‐ ( 4 ‐fluoro ‐ 3 ‐ phenoxyphenyl ) ‐ methly ‐ 3 ‐ ( 2,2 ‐ dichloroethenly ) ‐ 2,2 ‐dimetylcyclopropancarboxylate ], imperator [ cyano ‐ 3 ‐ phenoxy ‐ benzyl ‐ cis,trans‐3 ‐ ( 2,2 ‐ dichloroethenyl ) ‐ 2,2 ‐ dimetylcyclopropancarboxylate ] , Meothrin [ cyanophenoxybenzyl ‐ 2,2 3,3 ‐ tetramethy 1 cyclopropanecarboxylate] ,Sumicidin [ cyano ‐ 3 ‐ phenoxybenzylcyclopropane carboxylate ] T C A (trichloroacetic acid ) and talstar. I₅₀ values of chemicals caused inhibition were determined by means of activity percantage [ I ] diagrams. Pesticides having the strongest inhibitory effects on each enzyme were found to be talstar ( I₅₀ =7,3×10⁻⁴ M) for ALK‐P ; talstar ( I₅₀ =7,1×10⁻⁴ M) and baytroid (I₅₍₎ =8,8×10⁻⁴ M) for amylase; baytroid (I₅₀ =1,25×10⁻⁴ M) for CK; baytroid ( I₅₀ =8,8×10⁻⁴ M and I₅₀ =9,7×10⁻⁴ M) for GGT‐P and LDH. Baytroid and talstar in general caused a high inhibition for all enzymes. Pollutants such as TCA, meothrin, imparator, sumicidin weakly inhibed each enzymes.

Key words:

• Pesticides
• inhibitor
• serum enzymes