Deodorization of pig manure using lignin peroxidase with different electron acceptors

ABSTRACT

Odor pollution is a big environmental problem caused by large-scale livestock production in China, and developing a practical way to reduce these odors is pressing. In this study, a combination of 0.2–1.0 U/mL lignin peroxidase (LiP) and one of three peroxides (H₂O₂, CaO₂, 2Na₃CO₃·3H₂O₂) was examined for its efficiency in reducing the release of eight chemicals (propionic acid, isobutyric acid, isocaproic acid, isovaleric acid, phenol, p-cresol, indole, and skatole), NH₃, H₂S, and odor intensity from pig manure. The results showed an approximately 90% reduction in p-cresol, 40–60% reduction in odor intensity, 16.5–40% reduction in indolic compounds, and 25–40% reduction in volatile fatty acids. Being the electron acceptors of LiP, 2Na₃CO₃·3H₂O₂ and CaO₂ performed better than H₂O₂ in reducing the concentration of eight chemicals, NH_3, H₂S, and odor intensity from pig manure. The effect of deodorization can last for up to 72 hr.

Implications: In China, one of the major environmental problems caused by confined feeding is odor pollution, which brings a major threat to the sustainability, profitability, and growth of the livestock industry. To couple the LiP with the electron acceptors, a low–cost, simple, and feasible method for odor removal was established in this study. Based on the study results, a practical treatment method was provided for odor pollution and supply the farm operators a more flexible time to dispose treated manure.

Additional information

Funding

This work was supported by the International Science & Technology Cooperation Program of China (2013DFA61260), the Key Project of Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-15-02; KSCX2-EW-G-15-01), and the Chinese Academy of Environmental and Application of Microbial Key Laboratory Fund Project (KLCAS-2013-05).

Notes on contributors

ZhiYing Yan

ZhiYing Yan and XiuFeng Liu are professors at the Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Environmental Microbiology Key Laboratory of Sichuan Province.

XiuLi Wei

YueXiang Yuan, Dong Li, and ZhiDong Li are associate professors at the Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Environmental Microbiology Key Laboratory of Sichuan Province.

YueXiang Yuan

XiuLi Wei is an associate professor at Chongqing Academy of Agricultural Sciences.

ZhiDong Li

YueXiang Yuan, Dong Li, and ZhiDong Li are associate professors at the Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Environmental Microbiology Key Laboratory of Sichuan Province.

Dong Li

YueXiang Yuan, Dong Li, and ZhiDong Li are associate professors at the Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Environmental Microbiology Key Laboratory of Sichuan Province.

XiuFeng Liu

ZhiYing Yan and XiuFeng Liu are professors at the Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Science, Environmental Microbiology Key Laboratory of Sichuan Province.

LiHong Gao

LiHong Gao is a professor at Chongqing Academy of Agricultural Sciences.