Comparative in vitro antimicrobial activities of CSA-142 and CSA-192, second-generation ceragenins, with CSA-13 against various microorganisms

Abstract

Ceragenins, a novel family of antibiotics, are cationic derivatives of cholic acid and display broad-spectrum antimicrobial activities. Multiple ceragenins have been synthesized and studied, and most published data are with ceragenin CSA-13. This study aimed to investigate the in vitro antimicrobial properties of second-generation ceragenins, CSA-142 and CSA-192, and compare them to CSA-13. The antimicrobial activities of CSA-13, CSA-142 and CSA-192 were studied against 20 strains of Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Time-kill curve methods were performed to determine the bactericidal and fungicidal activities of the ceragenins against three strains of each microorganism. Overall, CSA-13 showed the lowest MIC values, but also CSA-142 and CSA-192 had good activities against tested microorganisms. The killing curves showed that ceragenins generally had bactericidal and fungicidal effects. The bactericidal and fungicidal behaviours of CSA-142 and CSA-192 may make them good alternative agents to CSA-13.

Keywords:

• Ceragenins
• Dynamic time-kill experiments
• In vitro activities

Conflicts of interest

No potential conflict of interest was reported by the author.

Additional information

Notes on contributors

Cagla Bozkurt Guzel

All authors have contributed to the study and approved the manuscript. This work was supported by a grant from the Research Fund of the University of Istanbul (Istanbul, Turkey). Project number: TYD-2017-24366.

Ozlem Oyardi

All authors have contributed to the study and approved the manuscript. This work was supported by a grant from the Research Fund of the University of Istanbul (Istanbul, Turkey). Project number: TYD-2017-24366.

Paul B. Savage

All authors have contributed to the study and approved the manuscript. This work was supported by a grant from the Research Fund of the University of Istanbul (Istanbul, Turkey). Project number: TYD-2017-24366.