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Summary
The appearance of enterochromaffin cells in the intestine of chick embryo was studied by means of technics suitable to give argyrophil and argentaffin reaction. Argyrophil reaction first appears in duodenal cells at 14th day of incubation; the argentaffin reaction appears only at 16th day. These findings agree with Ersparmer and Vialli's theory, according to which the appearance of enterochromaffin substance goes through two successive stages: the first caracterized by argentophil reaction positivity, the second by the argentaffin one.