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COST 8.22 Development of integrated systems for large-scale propagation of elite plants using in-vitro techniques

Characterization of genome variation in tissue cultures by RAPD fingerprinting — A methodological comment

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Pages 115-120 | Received 22 Feb 2000, Accepted 12 Sep 2000, Published online: 05 Aug 2006
 

ABSTRACT

Genome variation in tissue culture is of importance for commercial use in plant propagation as well as for basic research on plant growth and development. RAPD fingerprinting can be used to trace genetic or epigenetic changes at the genome level. In the present paper, results of RAPD analyses on primary tissue cultures are given with particular attention to the repeatability of the method. The significance of primer binding site competition for the discovery of qualitative and quantitative DNA polymorphism is discussed.

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