Growth, water relations and antioxidant defence mechanisms of olive (Olea europaea L.) subjected to Partial Root Drying (PRD) and Regulated Deficit Irrigation (RDI)

Abstract

We compared the effects of Partial Root-zone Drying (PRD) and Regulated Deficit Irrigation (RDI) on water relations, vegetative growth and antioxidant enzyme activities in two olive varieties Picholine marocaine (Pm) and Picholine languedoc (Pl). A split-root technique was used to divide the root system of the plants in two parts, placed in separate pots, and exposed simultaneously to different water regimes: (i) Control with both root compartments well-watered, (ii) PRD, with one compartment fully irrigated, while the other was kept dry, and (iii) RDI, with both compartments partially irrigated. Compared with the control, both PRD and RDI treatments resulted in decreased stomatal conductance (Gs), pre-dawn leaf water potential (ψ_pd) and relative water content (RWC). The PRD-treated plants of both varieties exhibited lower Gs, and higher ψ_pd and RWC compared with those exposed to RDI, although both treatments received the same amount of water. Plant vegetative growth was substantially reduced under both PRD and RDI compared with the control, as expressed by lower values of shoot length, leaf number and total leaf area. The enzymatic activities of superoxide dismutase, soluble peroxidase, insoluble peroxidase, and polyphenol oxidase were up-regulated by water deficits under PRD and RDI treatments, compared with the control.

Key words:

• Olive
• irrigation
• partial root drying (PRD)
• regulated deficit irrigation (RDI)
• stomatal conductance
• water status
• vegetative growth
• antioxidant enzymes

Acknowledgements

This research was financially supported by the EU-INCOMED project IRRISPLIT (ICA-1999-100008). The meteorological data presented in Figure 1 were obtained by the meteorological station of the IRD/ Sud-Med project co-ordinated by the Dr AG Chehbouni, CESBIO, Toulouse, France.