In vitro plant regeneration from leaf callus of Grindelia robusta Nutt

Abstract

A regeneration protocol from leaf explants of Grindelia robusta Nutt. was developed. The combination of 0.5 mg l⁻¹ IBA plus 0.5 mg l⁻¹ or 1 mg l⁻¹ BA added to Murashige-Skoog (MS) medium resulted in the best callus induction frequency; the combination of 0.4 or 0.9 mg l⁻¹ BA plus 1.2 mg l⁻¹ GA₃ resulted in the best shoot regeneration. Rooting was successful on MS medium supplemented with 0.5 mg l⁻¹ IBA. Hardening of G. robusta plants was accomplished in 30 days with 85% survival rate.

Keywords:

• Callus culture
• Grindelia robusta
• growth regulators
• plant regeneration

Acknowledgements

The authors are grateful to Dr. Filippo Bedini for technical assistance.