Effect of CPPU on in vitro axillary shoot proliferation and adventitious shoot regeneration in kiwifruit

Abstract

The effect of CPPU, N‐(2‐chloro‐4‐pyridyl)‐N‐phenylurea, on the development of axillary buds and on adventitious shoot regeneration was investigated in mature leaves of in vitro‐cultured shoots of Actinidia chinensis Planch (Sel. K190) and Actinidia deliciosa A. Chev. cultivars Hayward and Tomuri. In the multiplication phase, 2.2 mM CPPU induced proliferation rates comparable to 4.4 mM benzyadenine (BA) both in Hayward and in Tomuri, while a higher CPPU concentration reduced proliferation. In A. chinensis, significant differences in multiplication rates were not detected between BA and CPPU. However, shoots developed on CPPU appeared hyperhydric, and had very short internodes, reduced leaf laminas, higher water, carotenoid and phenol contents and considerably lower chlorophyll level in comparison with the BA‐grown shoots. On the other hand, in adventitious shoot regeneration CPPU was more effective than zeatin (ZEA) and BA in A. deliciousa cultivars and the best morphogenic response was obtained with the lowest concentration (10 mM) in cv Hayward, while 16 mM was the most efficient treatment in cv Tomuri. In A. chinensis, CPPU was as efficient as ZEA when the highest concentration was used.

Abbreviations:

• BA, 6‐benzyladenine
• CPPU, N‐(2‐chloro‐4‐pyridyl)‐N‐phenylurea
• IBA, indole‐3‐butyric acid
• MS, Murashige and Skoog
• WC, water content
• ZEA, zeatin

Keywords:

• Actinidia chinensis Planch
• Actinidia deliciosa A. Chev
• carotenoids
• chlorophyll
• cytokinins
• phenols
• shoot morphogenesis

Acknowledgements

The authors thank Loreta Marinaccio for the skilful assistance in subculturing the in vitro material.