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ABSTRACT
A specimen of Orchis x dietrichiana, a natural hybrid between O. tridentata and O. ustulata (Orchidaceae) was collected at flowering time on the mountains E of Vallo di Diano (Salerno province, Italy) and characterised by using morphology and molecular characters. Randomly Amplified Polymorphic DNA (RAPD) and Restriction Fragment Length Polymorphism (RFLP) analysis of plastidial DNA (cpDNA) data were employed with the aim of gathering information on the hybrid nature of O. x dietrichiana and of verifying the hypothesis formulated for its parental lineage. RAPD showed that the plant is indeed a hybrid between the two above-mentioned species and the cpDNA pattern of the hybrid clearly demonstrated that O. tridentata provided the maternal lineage.
