Establishment of Aster sedifolius and Aster caucasicus callus cultures as a potential source of antioxidants

Abstract

Callus cultures were established for Aster sedifolius and Aster caucasicus, two Aster species used in natural medicine for their anticancer, antibacterial and antiviral activities attributed to the high content of antioxidant compounds such as polyphenols and ascorbate. The effects of growth medium and light condition on the induction and growth rate of callus from leaf, petiole and root explants are reported. Callus induction and proliferation depended on the genotype and the experimental conditions. In particular, a profuse callus culture was obtained from leaf explants grown in the light on medium supplemented with 2,4-D (0.1 mg l⁻¹) for A. caucasicus and on medium supplemented with 2,4-D (0.44 mg l⁻¹) plus 6-benzil-ammino-purine (BAP) (0.22 mg l⁻¹) for A. sedifolius. The content of total polyphenol and ascorbic acid was estimated in leaf and petiole explants of in vivo plants and in the relative derived calli. In calli, polyphenol content was lower than in the corresponding in vivo organs. Furthermore, the total ascorbic acid content decreased in calli while the reduced ascorbic acid pool increased. These findings demonstrate that Aster callus cultures produce antioxidant compounds and as such might be a model system to investigate the regulation and production of these important metabolites.

Keywords:

• Ascorbic acid
• in vitro culture, polyphenols

Acknowledgments

The authors thank Dr. Di Matteo Antonio for assistance in metabolites analysis and Mrs. Lotti Elvira for her technical assistance. This work was supported by a grant from the Ministry of Agricultural, Food and Forestry Policies (MiPAAF), Italy, as part of the BIOPEST project and contribution from DISSPAPA n. 218 is also acknowledged.

Additional information

Notes on contributors

Maria Minutolo

The first two authors contributed equally to this work.