Leaf anatomy and micromorphology of the Capparis spinosa (Capparaceae) group in Iran

Abstract

The genus Capparis L. (Capparaceae) comprises edible plants and species with medicinal properties. Capparis spinosa is widely distributed in Europe, Africa, Asia and Oceania. This taxon forms a polymorphic species complex within that, several species and infraspecific taxa have been identified. We have focused on the leaf anatomy and micromorphology of Capparis taxa in Iran to find efficient diagnostic characters. Fresh samples of different taxa studied of C. spinosa were collected. Leaf-blade cross-sections and leaf surface were considered. Multivariate statistical analyses were used for illustrating the species and varieties similarities. Leaf anatomical features, such as midrib vascular bundle width and length, abaxial and adaxial epidermis thickness, abaxial and adaxial cuticle thickness, stomata depth, midrib outline on the adaxial surface, epicuticular wax, stomata average length, stomata number per leaf area and trichome type on the leaf surface were of diagnostic importance. As there is some degree of taxonomic confusion between different varieties of C. spinosa and mainly herbarium samples can provide stems and leaves for analyses, our anatomical study tries to overcome this problem. Results showed that selected features are of taxonomic importance in species and varieties separation.

HIGHLIGHTS

• Leaf anatomy and micromorphology provided useful information to distinguish taxa within the C. spinosa group.

• C. cartilaginea with its thick leaves shows a distinct position from other taxa studied.

• Multi-layers of palisade parenchyma and occurrence of stomata on both sides of the leaf enable C. spinosa to increase its photosynthetic rate and decrease its temperature during drought conditions.

Keywords:

• Anatomy
• micromorphology
• Capparis
• Iran
• similarity

Acknowledgments

We thank curators of the MIR, IRAN and TUH herbaria for providing us the specimens to study. We also thank the Iran national science foundation for their supports.

Disclosure statement

The authors declare that they have no conflict of interest.

Additional information

Funding

This work was supported by Iran National Science Foundation [grant number: 97009938].