Mitigation of salt stress and stimulation of growth by salicylic acid and calcium chloride seed priming in two barley species

Abstract

The main objective of the current study is to underline the effects of salicylic acid or calcium chloride seed priming on plant growth, water content, chlorophyll content and photosynthetic activity of two barley species Hordeum marinum and Hordeum vulgare exposed to salinity (0, 100 and 200 mM NaCl). Results revealed that salinity decreased shoot and root growth, transpiration rate, chlorophyll concentration, stomata conductivity and water content in plants obtained from unprimed seeds. This reduction was dependent on the saline concentration used. However, Hordeum marinum species showed tolerance by maintaining tissue hydration, growth, photosynthetic assimilation and chlorophyll biosynthesis in stressed plants compared to controls. Moreover, priming seeds with salicylic acid or calcium chloride significantly improved all the above parameters under different salt treatments, suggesting that the effectiveness of this approach mainly depends on the priming agent applied and may vary with species and stress intensity applied.

Keywords:

• Barley
• seed priming
• salinity
• photosynthetic
• improvement
• tolerance

Acknowledgements

This work was conducted in the Laboratory of Extremophile Plants (LPE: Laboratoire des Plantes Extrêmophiles, Tunisia) of the Centre of Biotechnology of Borj-Cédria (CBBC: Centre de Biotechnologie de Borj- Cédria, Tunisia), and supported by the Tunisian Ministry of Higher Education and Scientific Research (LR15CBBC02). The authors thank the staff of the Center of Biotechnology of Borj-Cédria (CBBC) for technical and administrative supports.

Credit authorship contribution statement

Rim Ben Youssef: performed the experiments, formal analysis, data interpretation; statistical analysis, Writing - original draft. Nahida Jelali: data analysis, co-wrote the manuscript. Nadia Boukari: helped in experiments. Chedly Abdelly: Supervision.

Disclosure statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.