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Original Articles

Osservazioni sul rapporto fra accumulo protoplasmatico del fluorocromo uranina e intensità respiratoria in Oscillatoria irrigua Kütz

Pages 89-97 | Received 27 Jul 1961, Published online: 14 Sep 2009
 

Abstract

OBSERVATIONS ON THE RELATION BETWEEN PROTOPLASMIC ACCUMULATION OF THE FLUOROCHROME URANIN AND RESPIRATORY INTENSITY IN OSCILLATORIA IRRIGUA Kütz. — Uranin (sodic fluorescein) is a vital dye of peculiar cytophysiological interest, since it is accumulated chiefly in the nucleus, cytoplasm and chondriosomes of living plants cell. The degree of accumulation of this dye is in evident relation with the cellular activity.

In the present paper the relation between respiration and secundary fluorescence induced by uranin in the cells of Oscillatoria irrigua is demonstrated. The A. has also observed that a reduction of the respiratory activity of so coloured cells through inhibitory substances as f. i. oxyquinoline results in a releasing of uranin from the cell. In this case the dye is no more detained in the cells and flows outwards.

The true nature of the accumulation of uranin and its relations with the cellular activity are not yet clear, but its value as research mean to appreciate the cellular activity of organisms is emphasized.

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