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Abstract
INTRACELLULAR LOCALIZATION OF CHLOROGENIC ACID OXIDASE IN AGED POTATO TUBER DISCS. — The localization in the cells of chlorogenic acid oxidase has been investigated in potato tubers and discs of potato tuber. It has been ascertained that the rise of activity per gram of tissue, after preparation of discs, is not due to bacterial or fungal growth. The activity is widely distributed among cell fractions. Some activity is found in mitochondria, while most of the activity is distributed among soluble fraction and a « microsomal » fraction sedimented by centrifugation in the range 15.000–50.000 x g. This fraction appears to contain mitochondrial fragments, fragments of the endoplasmic reticulum and ribosomes. When tuber discs are aged, the rise of chlorogenic acid oxidase activity is much larger in the soluble than in particulate fraction.