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Abstract
Pinus Pinea ovules cultured in vitro. — The degree of growth and autonomical differentiation of Pinus pinea L. proembryo has been studied by means of controlled cultures in vitro of excised ovules.
Proembryos in vitro undergo involution and initials of their growth points change back into parenchimatoides cells.
Completely differentiated embryos cultivated in vitro behave as if they were not physiologicaly ripe in all their parts. Embryos cultured in august, september and october develop into rocotless seedlings. Only embryos cultured in november have roots able to elongate where germinating, but in a still scarce degree in comparison with hypocotile and cotiledons. Hypocotil root ratio is inverted as regards what happens in nature.
The primary endosperm of Pinus pinea L. cultivated in vitro undergoes surface diffuse proliferation.
A case of polyembriony has been observed.