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Abstract
A simple procedure for the biosynthetic preparation of carbon-C11 labelled D-glucose and D-fructose. — C 11-uniformely-labelled D-glucose and D-fructose have been prepared by exposing to C 11 O 2 leaves of Beta vulgaris var. saccharifera and Nicotiana tabacum. From 24 to 36 percent of the radioactivity supplied to the leaves was recovered in the two monosaccharides. The specific activity of D-glucose ranged from 8.7 to 18 microcuries per micromole while values from 8.3 to 20.8 were obtained in the case of D-fructose. The higher specific activities were always obtained when N. tabacum was employed. As compared to the radioactive monosaccharides further purified by two-dimensional paper chromatography, D-glucose and D-fructose prepared according to the proposed procedure showed a radiochemical purity of 98.7 and 99.8 percent, respectively.
After exposure to C 14 O 2 (final concentration 0.345 percent, by volume) for 24 hours at room temperature and 700 lux, the leaves were homogenized with quartz and extracted with water and 80 percent ethanol. The sugars were purified first by chromatography on a column of cocoanut charcoal, then by preparative paper chromatography. The time required for a complete experiment is of approximately 17 hours.
The procedure may be conveniently applied to preparations employing from a few microcuries up to 100 millicuries of BaC 14 O 3. No special equipment is required.