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Nutrition and Food Safety Section

SCREENING AND CONFIRMATORY DETERMINATION OF CLENBUTEROL RESIDUES IN BOVINE MEAT MARKETED IN THE NORTHWEST OF MEXICO
MONITOREO Y CONFIRMACIÓN DE LA PRESENCIA DE RESIDUOS DE CLENBUTEROL EN CARNE DE BOVINO COMERCIALIZADA EN EL NOROESTE DE MÉXICO

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Pages 130-136 | Received 21 Jan 2008, Accepted 24 Mar 2008, Published online: 02 Oct 2009
 

Abstract

Resumen

It is essential from the public health point of view, that the Mexican regulatory agencies implement a monitoring surveillance program that not only uses preliminary screening methods for the determination of clenbuterol, but also uses confirmatory methods such as MS-MS techniques. Thus, the aim of this work was to monitor the presence of clenbuterol residues in bovine meat muscle collected from the local market. From 50 bovine meat samples analyzed by the ELISA test, 6 (12%) presented clenbuterol residues from 3.06 to 6.12 μg/kg, 13 (26%) from 0.5 to 1.83 μg/kg, 24 (48%) from 0.1 to 0.5 μg/kg and only 7 (14%) were below the limit of detection (LOD) of 0.1 μg/kg. The six samples with the highest clenbuterol residues were subjected to GC-MS-MS for confirmation. The results showed that clenbuterol was illegally used as a bovine growth promoter.

El objetivo del presente trabajo fue determinar y confirmar la presencia de residuos de clenbuterol en carne de bovino comercializada en el noroeste de México. De un total de 50 muestras de carne de bovino obtenidas del mercado y analizadas por inmunoensayo específico ligado a enzima (ELISA), 6 muestras (12%) presentaron residuos de clenbuterol en un rango de 3,06 a 6,12 μg/kg, 13 muestras (26%) de 0,5 a 1,83 μg/kg, 24 muestras (48%) de 0,1 a 0,5 μg/kg y sólo 7 muestras (14%) se encontraron por debajo del límite de detección (LOD) de 0,1 μg/kg del ensayo ELISA. Las 6 muestras que presentaron los más altos residuos de clenbuterol, fueron sujetas a confirmación por medio de cromatografía de gases acoplada a un detector selectivo de masas-masas (GC-MS-MS). Los resultados encontrados confirmaron la presencia de clenbuterol, el cual fue ilegalmente utilizado como promotor de crecimiento de bovinos.

Palabras clave: Residuos de clenbuterol, carne de bovino

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