Identification and characterization of male specific protein in the hemolymph of Galleria mellonella L.

Abstract

Male specific protein (MSP) was identified and purified from the hemolymph of Galleria mellonella L. by electrophoresis and anion exchange chromatography. MSP has a native molecular weight of 55 kDa as determined by gel filtration chromatography and consists of a single unit with the apparent molecular weight of 27 kDa and has the pl of approximately 5.8. MSP is present in the hemolymph from day 8 pupae throughout the male adult. MSP was also found in pupal fat body, adult fat body, and adult testis.

Key words:

• Galleria mellonella L.
• Hemolymph
• Male specific protein
• Western blotting
• Fat body
• Testis

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