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Acta Botanica Gallica
Botany Letters
Volume 155, 2008 - Issue 3
489
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Original Articles

Rapid plant regeneration and assessment of genetic fidelity of in vitro raised plants in Aloe barbadensis Mill. using RAPD markers

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Pages 427-434 | Received 25 Oct 2007, Accepted 27 Nov 2007, Published online: 26 Apr 2013
 

Abstract

Rapid micropropagation was achieved in Aloe barbadensis Mill. using shoot meristems as explants. Random Amplified Polymorphic DNA (RAPD) markers were used to determine the genetic fidelity of in vitro raised plants. Forty decamers were used to amplify DNA from in vitro and in vivo donor plants to assess the genetic integrity. All RAPD profiles from in vitro raised plants were monomorphic and similar to that of field grown donor plants. No variation was detected within the in vitro raised plants. High multiplication frequency and molecular stability ensure the efficacy of the protocol developed for the production and conservation of this important medicinal plant.

Résumé

Une micropropagation rapide a été réalisée sur des pousses méristématiques d'Aloe barbadensis Mill. Des marqueurs RAPD (Random Amplified Polymorphic DNA) ont été utilisés pour déterminer la fidélité génétique des plants produits in vitro. Tous les profils RAPD issus des plants cultivés in vitro sont monomorphiques et semblables aux plants mères cultivés en champ; aucune variation n'y a été détectée. De hautes fréquence multiplicative et stabilité moléculaire assurent l'efficacité du protocole développé pour la production et la conservation de cette importante plante médicinale.

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