Abstract
This work investigates the combined effects of N form and Cd treatment on photosynthesis of tomato (Solanum lycopersicum Mill. cv Ibiza F1). Our study shows that, compared to plants fed with NO3“, dry weight, fresh weight and leaf area decrease when plants received NH4 + as N form. Regardless the N form used, Cd inhibits this parameters. In this report we show that, when grown in NH4 +, Cd—stressed seedlings accumulate higher levels of chlorophyll. Under Cd stress, water content, transpiration rate (E), stomatal conductance (Gs) and photosynthetic activity (Amax) decreased in plants fed with NO3 − and increased in NH4 +- grown-plants. The Fv/Fm ratio of dark adapted leaves was lower in NH4+- grown tomato compared to in NO3“-grown seedlings. Upon exposure to metal, the Fv/Fm ratio and the fraction of open PSII reaction centers (qp) were depressed by Cd in NO3“−grown plants and enhanced in NH4 +-grown ones. NPQ stimulated by Cd in NO3 −-grown plants was inhibits in NH4 +-grown ones.
Résumé
Ce travail examine les effets combinés de la forme d'azote et du traitement par le cadmium sur la photosynthèse de la tomate (Solanum lycopersicum Mill. cv Ibiza F1). Par comparaison aux plantes recevant NO3“, la matière sèche et fraîche ainsi que la surface foliaire diminuent chez les plantes cultivées en présence de NH4 +. Quelle que soit la forme d'azote utilisée, Cd inhibe ces paramètres. Les plantes stressées par Cd et cultivées en présence de NH4 + accumulent plus de chlorophylle. Sous stress cadmique, les teneurs en eau, la transpiration (E), la conductance stomatique (Gs) et l'activité photosynthétique (Amax) diminuent chez les plantes recevant NO3 − et augmentent chez celles cultivées en présence de NH4 +. L'efficience maximale des feuilles adaptées à l'obscurité (Fv/Fm) est plus faible chez les plantes cultivées en milieu ammoniacal que chez celles cultivées en milieu nitrique. Suite à l'exposition au métal, Fv/Fm ainsi que la fraction de centres de réaction PSII ouverts (qp) diminuent chez les plantes recevant NO3 − et augmentent chez celles recevant NH4 +. Par contre, la désaltération non photochimique (NPQ) est stimulée par Cd chez les plantes recevant NO3 − et inhibé chez celles issues du milieu ammoniacal.
Abreviations
| N | = |
nitrogen |
| Cd | = |
cadmium |
| Amax | = |
photosynthetic rate |
| E | = |
transpiration rate |
| Gw | = |
stomatal conductance |
| Ci | = |
intercellular CO2 concentration |
| Fv/Fm | = |
maximum quantum efficiency of PSII photochemistry |
| qp | = |
photochemical quenching |
| NPQ | = |
nonphotochemical quenching |
| ΦPSII | = |
PSII efficiency |
| Qexc | = |
efficiency of excitation energy capture by open PSII reaction centres |
| QA | = |
primary quinone electron acceptor of PSII |
| PSII | = |
photosystem II |
Related Research Data
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