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ABSTRACT
Interploidy gene transfer in Avena is difficult because of the lack of homology and lack of pairing between chromosomes in diploid, tetraploid, and hexaploid oat species. In three Wisconsin projects, 6x amphiploids, a derived tetraploid, and a natural tetraploid were used as donor parents for resistance to crown rust [Puccinia coronata Cda. f. sp. avenae (Fraser and Ledingham)] or stem rust [Puccinia graminis Pers. f. sp. avenae (Eriks and E. Henn)J. Multiple backcrosses to susceptible A. sativa parents while constantly selecting for rust resistance resulted in the derivation of unstable, rust resistant, monosomic alien substitution (MAS) lines. Seeds from resistant MAS plants were irradiated in attempts to induce translocation of the gene for resistance from the alien chromosome to an A. sativa chromosome such that the gene would pass in normal fashion through male gametes. The sequence of irradiation, screening for resistant plants to be used as male parents, and crosses to susceptible cultivars as females resulted in the derivation of stable, resistant translocation lines in each of the three projects.