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ABSTRACT
All possibilities for stable diagnostic of strong infection and economicaly important for Carnation mottle virus have been explored. Comparative tests on the sensitivity of some different variants of ELISA have been carried out—direct and indirect by Clark and Adams (1), Lommel et al. (6) and Edwards and Cooper (2). There are used alkaline phosphatase and peroxidase conjugates. The conditions for the different tests to be carried out are optimized—concentrations of immunoglobulins (IgG) and conjugates, the durations of incubation of antibody and antigene. The comparative observation on ELISA variants shows that DAS ELISA is the most sensitive—1 ng/ml by using the peroxidase conjugate. Carnation mottle virus (CaMV) can be identified in maximum diluted sap - 1:500 000, as by alkaline phosphatase and peroxidase conjugate as well. The second is preferable because of its low cost.
The indirect variants are with less sensitivity. By the method of Lommel et al. (6) 10 ng/ml can be detected and that quantity of virus is identified by two conjugates enough all right. By the method of Elwards and Cooper (2) 1 ng/ml can be detected by using antisera instead of IgG and long time of substrate incubation - 16 hours.
On the base of experimental series carred out for practical applications in the process of production of healthy carnation plants DAS ELISA with peroxidase conjugate is recommendible as the most sensitive and effective method.