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Original Articles

КОЛИЧЕСТВЕНО ОПРЕДЕЛЯНЕ НА ЧОВЕШКИ ПЛАЗМЕН ФИБРОНЕКТИН ЧРЕЗ ИМУНОЕНЗИМЕН МЕТОД С МОНОКЛОНАЛНО АНТИТЯЛО

КОЛИЧЕСТВЕННОЕ ОПРЕДЕЛЕНИЕ ЧЕЛОВЕЧЕСКОГО ПЛАЗМЕННОГО ФИБРОНЕКТИНА С ПОМОЩЬЮ ИММУНОФЕРМЕНТНОГО МЕТОДА МОНОКЛОНАЛЪНОГО АНТИТЕЛА

QUANTITATIVE DETERMINATION OF HUMAN PLASMA FIBRONECTIN BY IMMUNOENZYME METHOD WITH MONOCLONAL ANTIBODY

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Pages 27-31 | Published online: 15 Apr 2014
 

РЕЗЮМЕ

Описан иммуноферментный метод количественного определен и фибронектина в человеческой плазме. Установлены оптимальные условия тест а - системы: дл сенсибилизации твердоlt фазы желатиновым раствором в коли- честве 100 μg/ml, разведен и плазмы в соотношении 1:100 и ее инкубации в течение двух часов при 37°С. Вариации ивтра- теста—6 %, а китеротеста- 7.2 %. Основные биореагенты - моноклональное антитело и очищенный фибронехтин отечественного производства. Методика апробирована ва 34 здоровых и 128 больных пациентах с различными заболеваниями.

SUMMARY

The aim of this work was to develop an immunoenzyme method for quantitative determination of Fn in human plasma. We evaluated the optimal conditions of the test system: the gelatin solution for coating of the solid phase was 100 μg/ml, samples were tested in 1:100 dilution and incubated for 2 h at 37°C. Intra - test variation was 6 % and inter - test variation wets 7.2%. The main reagents - monoclonal antibody and purified fibronectin used as standart were Bulgarian products. The method was applied to 34 healthy subjects and 128 patients with different diseases.

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