ABSTRACT
Direct enzyme immunoassays (ELISA) for determination of zeatin- and isopentenyladenine-cytokinins were developed. The assays are sensitive and measuring ranges extend from 0.5 to 75 pmol for zeatinriboside and from 0.5 to 50 pmol for isopentenyladenosine. The accuracy and specificity of ELISA were checked by LC-MS. Direct enzyme immunoassay (ELISA) for indole-3-acetic acid determination was developed. The method was verified by HPLC with fluorometric detection.