In Vitro Culture and Somatic Embryogenesis of White Clover (Trifolium Repens L.)

ABSTRACT

A comparative examination in vitro of the morphogenetic characteristics of two cultivars and one candidate-cultivar of white clover (Trifolium repens L.) was carried out. Callus cultures were initiated from hypocotyl and cotyledon's tissues on five nutrient culture media. Embryo-like structures were generated in callus cultures only induced on MS nutrient medium containing 2 mg/l 2,4-D, 0.2 mg/l kinetin and 1 mg/l adenine. This medium turned out expediently balanced as for callus tissue initiation as well as for somatic embryoide induction. The complete development of differentiated structures to a plant was effectuated on MS culture medium with addition of 0.2 mg/l 2,4-D and 1 mg/l kinetin. In some of the obtained regenerants morphological deviations were established.