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ABSTRACT
The influence of new synthetic auxin hydrazide of 4-chlorophenoxyacetic acid (H-4-CPA) and cytokinin 1-(3-chlorophenyl)-3-[2-(4-methyl)pyridyl]-2-urea (3-CP-4-MPU) on the growth of callus tissues from tobacco was investigated. The substances were applied exogenously to the autoclaved nutrient medium independently and in combination at both concentrations: 0.5 mg/l and 4.0 mg/l. 3-CP-4-MPU (0.5 mg/l or 1.9 μ M) manifested higher growth stimulating effect than kinetin (0.2 μ M—standard I). Kinetin at concentration 1.9 μ M equimolar to that of the used phenylurea cytokinin inhibited the growth of tobacco calli. The high concentration of 3-CP-4-MPU (4.0 mg/l or 15.2 μ M) stronfly reduced the fresh and dry weight, therefore the phenylurea cytokinin is an inhibitor for this plant system at concentration 8-fold higher than that of kinetin. H-4-CPA increased the tobacco callus growth at both used concentrations. The simultaneous application of 1.9 μ M 3-CP-4-MPU and 2.5 μ M H-4-CPA to tobacco callus accelerated its growth while the higher concentrations of both compounds (15.2 μ M 3-CP-4-MPU + 20.0 μ M H-4-CPA) inhibited the biomass accumulation.