ABSTRACT
Two winter wheat (Tr.aestivwn L.) lines 8–61 and 148–133–14 were used in this study. Callus culture and gamma-ray treatment were combined for inducing new initial breeding material. As a result of the present investigation three lines with increased spike length were developed from 8–61 regenerants. For 148–133–14 regenerants a new form with a higher spike length and number of spikelets per spike was obtained. The generation of new forms depends both of gamma-ray treatment and genotype specificity.