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ABSTRACT
Rabbit an tisera against xylanase fraction (F-III-II-I) from Aspergillus oryzae with Mr 40 kDa were prepared. In double diffusion experiments, antiserum to this fraction formed a precipitation band. Western blotting analysis using antibodies against the xylanase showed that antibodies obtained from us recognized the main xylanase fraction and showed a single immunopositive protein with Mr of 40 kDa. The inhibitory effects of the antibody on xylanase activity were assayed. Maximum inhibition occurred at 0.72 mg/ml concentration of antibodies. Three enzyme preparations were tested against the antibodies from Aspergillus oryzae.