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ABSTRACT
Transgenic plants of carnation (Dianthus caryophyllus) were obtained by Agrobacterium mediated transformation. A regeneration procedure was based on the leaf and petals as initial explants. Two different phytohormones- BAP and NAA were employed for the production of adventitious shoots. Transformation of the cultivars Lena, Line 84 and Yanita was performed with a plasmid (p2260 1 170) containig marker genes—npt II and hpt genes encoding neomycinephosphotransferase and hygromicinephosphotransferase, respectively. For cvs. Scania, Nasslada and Lena the plasmid vip 104-carring anti-sense CHS-A and nptII was used. Cultivar Regina was transformed with plasmid vip 165- GUS gene under chs-J promoter. Transformation efficiency varied among tested cvs. Presence of the npt II gene in plants was confirmed by PCR and Southern blot analysis. The expression of CHS-A was confirmed by Northern blot.