Microspore Culture of Winter Oil Rapeseed (Brassica Napus): I. Effect of Culture Conditions on Embryo Formation from Microspores in Winter Oil Rapeseed

ABSTRACT

Embryogenie response of rapeseed microspore culture from ten hybrid combinations have been evaluated. The frequency of embryo formation was strongly influenced by genotype. Bud size correlated with microspore stage of development has been determined for any of studied genotype. Embryo formation occur when microspores are predominantly in late uninucleate stage of development. Our experiments suggest that optimal density for embryo induction is between 40000 to 60000 microspore per ml. The increased embryo induction rate have been achieved when growth hormones were included in culture media.