Purification and Separation of Individual Elastases from Streptomyces sp. 82

ABSTRACT

A elastolytic Streptomycetes strain was isolated from soil and was identified as Streptomyces sp. 82. This strain produced several kinds of proteases that the major constituent were elastases. Two elastases were purified by a combination of ion-exchange chromatography and gel- filtartion. Elastase—I had a molecular weight of 35 kDa and elastase II—28 kDa. Elastin, ATEE and BAEE were good substrates for these enzymes. Mixture of purified elastolytic fractions of Streptomyces sp. 82 were solubilized the leather wastes.