ABSTRACT
A toxin substance of Phytophthora parasitica var. nicotianae with 67kD Mw was purified from culture filtrate of the pathogen. The toxin was shown to have host specific toxicity in detached leaf bioassay at cultivar level. A ELISA system with polyclonal antibodies was established to quantify the toxin. The quantity of the toxin in the culture filtrate increased to the sixteenth day during the cultivation in the determinated conditions.