Analysis of Some Endogenous Plant Hormones During Induction of Somatic E Mbryogenesis in Common Bean (Phaseolus Vulgaris L.)

ABSTRACT

The contents of IAA and ACC in different explants like globular structures, residual explants and cultural media were investigated, during induction of somatic embryogenesis in common bean (Phaseolus vulgaris L.): variety Dobrudjanski 7 and the mutant one, Plovdiv 11M. Somatic embryogenesis was induced in liquid media B₅, containing 2,4-D in concentrations of I and 2 mg/l. The endogenous content of IAA varied in dependence of the genotype, explant's type and their age. The concentration of exogenous applied auxin 2,4-D and the endogenous content of IAA in the induced globular structures were inversely related. In spite of the exogenous auxin concentrations the embryos of Dobrudjanski 7 variety contained more IAA. With some exceptions, the residual explants were with low endogenous content of IAA. The concentration of 2,4-D and the content of IAA in the media, at the moment of somatic embryogenesis induction were positively related. The content of IAA was many times higher in embryos than in the residual explants and cultural media.