The Effect of NaCl on Protein Pattern of Somatic Embryos from Dactylis Glomerata L.

ABSTRACT

The effect of NaCl on the intracellular and ion-binding cell wall protein pattern and induction of isocatalases of salt-selected somatic embryos of Dactylis glomerata L. was studied. Differences observed in protein profiles could be indicative of physiological phenomena of salt adaptation. Salt-adapted callus cells showed an increased amount of cell wall-binding endohitinase and a decreased one for lipid-transfer protein. The pattern of intracellular isocatalases was modified under stress conditions evident from electrophoregrams. Two new isoforms were detected in salt-adapted embryos. The significant activity of these isoforms in adapted calluses indicated that they may be used as a marker for salt adaptation.