Effect of Inflorescence Length on the Callus Induction and Plant Regeneration in Orchardgrass (Dactylis Glomerata L.)

ABSTRACT

This study was carried out to determine the effect of inflorescence length on callus induction and plant regeneration in Orchardgrass (Dactylis glomerata L.). In the study, the inflorescences with different length (2–25 mm) isolated from the ecotype with a good in-vitro regeneration capacity were cultured on LS medium supplemented with 5 mg⁻¹ 2,4-D. Both callus induction and directly shoot regeneration from the inflorescences of orchard-grass were observed during the investigation. Callus induction rates varied from 50% to 84.5% depending on the inflorescence length. The highest callus weight per inflorescence was obtained from the inflorescence with 11–15 mm length. The inflorescences being longer than 15 mm produced lower callus weight than those being shorter than 15 mm. The highest regeneration rate (4.4 plantlets per inflorescence) was obtained from the inflorescences with the 11–15 mm length. Increasing the inflorescence length up to 20 mm decreased the regeneration rate. Considering the results of study, it was concluded that the inflorescences being yellowish in color and 6–20 mm length could be used as explant for an acceptable regeneration rate.