![Sample our Food Science & Technology journals, sign in here to start your access, latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5936/TOC-Subject-Sample-2021-Food-Science-Technology.jpg"})
ABSTRACT
Phosphatidylcholine-specific phospholipase C (PC-PLC) was purified 1875 fold from the culture medium of Pseudomonas fluorescens strain D. The purification procedure was based on the enzyme affinity to 2-(4-aminophenylsulphonyl)ethyl-cellulose. The purified PLC appeared as a single band after SDS PAGE. Pseudomonas fluorescens phospholipase C was terminally blocked. The sequence of the enzyme, secreted by P. fluorescens strain D apparently was not identical to that reported by Preuss et al. (24).