Effects of Genotype and Concentrations of Dicamba on Callus Induction and Plant Regeneration from Young Inflorescences of Perennial Ryegrass (Lolium Perenne L.)

ABSTRACT

This study was carried out to determine the effects of genotypes and dicamba concentrations (2.5, 5, 7.5 and 10 mg⁻¹) on the callus induction and plant regeneration from the segments of young inflorescences cultured on the LS-medium in perennial ryegrass (Lolium perenne L).

The result of the study showed that callus induction rate, callus weight per petri dish and plant regeneration from the young inflorescences were significantly affected by the genotypes. Depending on the genotypes, callus induction rate, callus weight mg/petri dish and number of regenerates per inflorescence segment varied from 20.3 %-67.2 %, 54.4–118.1, 0.797–2.719 respectively.

Callus and shoot induction rates, callus weight and regeneration rate were also significantly influenced by the dicamba concentrations. The segments cultured on the LS medium containing 5 mg⁻¹ of dicamba gave the highest values of callus induction rate (77.1 %), shoot induction rate (66.6 %), callus weight (168.2 mg/petri dish) and regeneration rate (3.458 regenerates per segment).