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ABSTRACT
The objective of this study was to determine the effects of genotype and Dicamba concentrations on the embryo culture of bread wheat (Triticum aestivum L.). Seven bread wheat cultivars were evaluated for their response to in vitro culture via culturing of their mature embryos as an explant in Linsmaier Skoog,(11) induction medium with different dicamba concentrations (2.5, 5, 7.5 and 10 mg−1). The results of the study showed that the frequency of responding embryo was significantly influenced by the genotypes and dicamba concentrations. Callus induction rate, callus weight per petri dish were significantly affected by the genotypes. Depending on the genotypes, callus induction rate, callus weight mg/petri dish varied from 16.7 %-85.4 %, 29.1–261.9 respectively.
Callus induction rate and callus weight were also significantly influenced by the dicamba concentrations. The cultured embryos on the LS medium containing 5 mg−1 of dicamba gave the highest values of callus induction rate (63.1 %) and also callus weight (155.1 mg/petri dish).