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ABSTRACT
It is aimed to determine the ultrastructural changes of testis that were formed in the testis of pupy rats which were bom from the mother rats with sialoadenectomy.
hi our investigation, 30 adult female Sprague-Dawley rats which had been weighted 250–350 gr were used. The female rats were divided into three equal groups of control (Sham), Sialoadenectomy (Sx) and Sialoadenectomy + Epidermal Growth Factor (Sx + EGF).
The rats in the control group were mated without any process had been made. The rats in the group of Sx were mated after sialoadenectomy and waiting for three weeks in order to decrease of the plasma EGF level.
After the sialoadenectomy it had been waited for three weeks and than the rats in the group of Sx+EGF were mated. On days 16–19 of pregnancy, a total of 50 microgram (μg) EGF was given to the animals in the same group with orogastric tub as 12.5 μg daily to each animal. The fetuses in each three group bom following pregnancy were kept to grow up until the 28th day. After 28 days the testes of male infant rats that had bom from three groups were fixed in buffered gluteraldehit of 2.5% and following this process the specimens were processed with routine histologic procedures and then were examined with TEM.
Seminifer tubules and Leydig cells of the rats in the control group were normal.
The basal membrans of seminifer tubules were thick and large intercellular spaces was established between the spermatogonia in the group of Sx. It was observed that Leydig cells were small and there was a dilatation between the cistemas of SER.
The basal membran of seminifer tubul in the Sx+EGF group was thick contrast to group of control however there was no intercellular space between the germ cells. While Leydig cells were found peritubuler localization and viewed normally, however a evident meiotic division was observed in spermatogonia.
In conclusion, exogen EGF had beneficial effect in the reversibility of the structural changes at the testes of the pupy rats depending on the absence of maternal EGF.