144
Views
1
CrossRef citations to date
0
Altmetric
Original Articles

Primary Asymmetric Division and Embryo Formation in a Single Cell Suspension of Embryogenic Medicago Falcata

, , &
Pages 27-33 | Published online: 15 Apr 2014
 

ABSTRACT

Single cell suspension culture of highly embryogenic line 47/1/150 M. falcata was initiated. The cell suspension was composed of different types of single cells. Most of the cells were capable to give rise to embryos following asymmetric division. In order to visualise and study this process and cells active and competent for division, transgenic Medicago falcata plants carrying the β-glucoronidase gene (gus A) under the control of the two promoters cdc2aAt and cyc3aAt from cell cycle regulating genes, have been obtained. Cell suspension cultures initiated from controls and transgenic plants were induced following the procedure for direct somatic embryogenesis in liquid medium. The fine fraction of single cells obtained after 15 days following culture initiation was used to investigate the asymmetry of the first cell division. The process of plant regeneration via somatic embryogenesis was characterised from single cell until conversion to plantlets.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.