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ABSTRACT
Eleven accessions of tobacco (Nicotiana tabacum L.) originating from Bulgaria are used to study the banding pattern of alcohol soluble proteins by polyacrylamide gel electrophoresis (PAGE). The structure and number of bands present in every genotype are observed. Four groups of bands are described according to the relative mobility from start position: low mobility bands (LMB); middle mobility bands (MMB); high mobility bands (HMB); fast bands (FB). High polymorphism is presented in MBB, where six types of banding are observed. Two genetically close variants selected within tobacco variety Djebel 576 are described as differing by protein spectra. The differences are assigned by the configuration of three protein groups. However tobacco seed protein spectra appear to have a relatively low level of genetic diversity as compared to cereals, this method is an opportunity for more comprehensive evaluation of genetic variants of tobacco at molecular level.