Registration of the Interaction Between C1q Human Complement Derivatives and Immunoglobulins by Elisa—Role of the Solid Phase

ABSTRACT

The interaction of C1q, the first subcomponent of the human complement, with its immunoglobulin ligands from immune complexes is the crucial step in the activation of the classical complement pathway. Thus the mechanism of these interaction and the factors, which influence them, are from high interest. In the present study the effect of immobilization of the interacting proteins on a solid support in ELISA was investigated. The obtained results lead us to the conclusion, that the immobilization process may have a significant influence on the binding activity of tested proteins, especially when hydrophobic interactions are involved.